| Bioactive Glass and Biopolymer Based Composite
Scaffold for Bone Regeneration |
Kanchan Maji and Sudip Dasgupta*
pages : 195-201
DOI : 10.1080/0371750X.2015.1092396 |
| Abstract |
| The composite scaffold of chitosan-gelatin reinforced with bioactive glass particles was prepared using freeze
drying method after blending chitosan and gelatin solution with bioactive glass where glutaraldehyde acted as a
cross-linking agent for biopolymers. Bioactive glass particles were synthesized using sol-gel route and characterized
using XRD and FTIR. The composite scaffold was prepared at a fixed solid loading of 30 wt% with a composition of
gelatin, chitosan and bioactive glass at 20:50:30 in weight. Characteristic IR band for (–C=N–) bond in the prepared
composite scaffold confirmed inter gelatin and chitosan-gelatin cross-linking in the scaffold with the help of
glutaraldehyde. Total porosity in the scaffold varied between 75% and 80% as determined by Archimedes principle.
Microstructural investigation using scanning electron microscopy revealed well interconnected porosity in the
scaffold with pore size in the range of 100-150 m. Bioactive glass particles were found to be well dispersed in the
pore wall of biopolymer matrix. The developed scaffold exhibited an average compressive strength of 2.48 MPa
which is close to the lower limit of compressive strength of spongy bone. In vitro bioactivity was assessed after
immersing the scaffold in simulated body fluid, with a result indicating an entire coverage of the scaffold with a
thick deposition of carbonated apatite after 10 days. MTT assay on the mesenchymal stem cells (MSCs) cultured
scaffold showed that the scaffold supported MSCs proliferation up to 7 days of cell culture. |
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